Epilepsy is a common seizure disorder affecting a wide array of animals and humans. Antioxidant/pro-oxidant imbalance are emerging as factors that contribute to the gradual process by which a normal brain develops epilepsy. These changes to the brain occasionally cause neurons to fire in a hyper-synchronous manner, known as a seizure. Medical ozone has been shown to reestablish redox balance and maintain the antioxidant/pro-oxidant balance that induce convulsions in animal models. As such, Holistic Veterinarians utilize ozone for seizure disorders and many other disorders where scientific support is substantial.


Influence of ozone’s treatments in mice submitted to PTZ-induced seizures was studied. Ozone was administered by rectal insufflation 1 mg/kg (5, 10, 15, 20 treatments), one per day, of 1-1·5 ml at an ozone concentration of 20 μg/ml. Mice received PTZ (90 mg/kg i.p.) 24 hours after the last ozone treatment. Oxygen control groups 26 mg/kg were introduced. Latency to first seizure was determined. Antioxidant/prooxidant balance in brain homogenates was studied. A1 adenosine receptors’ effects on ozone’s protective actions against seizures were evaluated using 8-cyclopentyl-1,3-dipropylxanthine (DPCPX).


Highest latency was observed when mice received 15 ozone treatments. Oxygen + PTZ group did not achieve protection against neither convulsions nor brain oxidative injury. Fifteen treatments of ozone protected against biomolecules oxidative damage and the antioxidant systems as well. 8-Cyclopentyl-1,3-dipropylxanthine abolished the ozone’s protection.(1)


Ozone therapy increased the latency for the first seizure and the survival percentage. These effects are discussed in point of ozone’s capacity to reestablish cellular redox balance, decrease biomolecules damage, and regulate activation of A1 adenosine receptors in PTZ-induced seizures.

Dr. Sarah Kalivoda
Mountain View Animal Hospital & Holistic Pet Care
Reno, Nevada


  • Neurological Research N 15 Mar;37(3):204-10. doi: 10.1179/1743132814Y.0000000445. Epub 2014 Sep 25.